fluorescence in situ hybridization

fluorescence in situ hybridization
Abbreviation: FISH
Hybridization of cloned, fluorescently labelled DNA or RNA, to intact biological materials, notably chromosome spreads and thin tissue sections. The technique allows the visualization of the physical location of nucleic acid sequences homologous to the probe, and is used for the placement of genes on chromosomes and for the spatial and temporal pattern of gene expression of specific mRNA molecules.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • fluorescence in situ hybridization — fluorescence in situ hybridization. См. нерадиоактивная гибридизация in situ. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Fluorescence in situ hybridization — A metaphase cell positive for the bcr/abl rearrangement (associated with chronic myelogenous leukemia) using FISH. The chromosomes can be seen in blue. The chromosome that is labeled with green and red spots (upper left) is the one where the… …   Wikipedia

  • fluorescence in situ hybridization — fluorescencinė hibridizacija in situ statusas T sritis augalininkystė apibrėžtis Chromosomų analizės metodas, kurį taikant naudojami švytintys fluorochromų žymekliai kaip alternatyva radioaktyviems žymekliams. atitikmenys: angl. FISH;… …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • fluorescence in situ hybridization — (= FISH; chromosome painting) Technique of directly mapping the position of a gene or DNA clone within a genome by in situ hybridization to metaphase spreads, in which condensed chromosomes are distinguishable by light microscopy. The DNA probe… …   Dictionary of molecular biology

  • fluorescence in situ hybridization — (FISH) in situ hybridization in which DNA probes are labeled with fluorescent tags and hybridized to metaphase or nondividing (interphase) chromosome spreads to identify and localize specific sequences. See also chromosome painting, under… …   Medical dictionary

  • fluorescence in situ hybridization — ▪ medicine       technique that employs fluorescent probes for the detection of specific deoxyribonucleic acid ( DNA) sequences in chromosomes (chromosome). FISH has a much higher rate of sensitivity and specificity than other genetic diagnostic… …   Universalium

  • fluorescence in situ hybridization — see FISH …   Medical dictionary

  • fluorescence in situ hybridization — see FISH …   The new mediacal dictionary

  • Fluorescent in situ hybridization — can be used to find out where the fluorescent probe bound to the chromosome. FISH is often used for finding specific features in DNA. These features can be used in genetic counseling, medicine, and species identification. Probes Probes are often… …   Wikipedia

  • nonradioactive in situ hybridization — nonradioactive in situ hybridization. = fluorescence in situ hybridization (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

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