purification tag
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affinity tag — An amino acid sequence that has been engineered into a protein to make its purification easier. The tag could be another protein or a short amino acid sequence, allowing purification by affinity chromatography. Synonym: purification tag … Glossary of Biotechnology
Protein-Tag — Als Protein Tag (engl. tag = Markierung) werden in der Biochemie verschiedene meist kurze Aminosäuresequenzen bezeichnet, mit deren Hilfe Proteine markiert werden können. Für verschiedene Zwecke sind verschiedenen Markierungen unterschiedlich gut … Deutsch Wikipedia
Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… … Wikipedia
Polyhistidine-tag — A polyhistidine tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N or C terminus of the protein. It is also known as hexa histidine tag, 6xHis tag, and by the trademarked name His tag… … Wikipedia
Tandem Affinity Purification — The Tandem Affinity Purification (TAP) is a technique for studying protein protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The protein of interest with the TAP tag first binds to beads… … Wikipedia
Protein tag — Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes … Wikipedia
his tag — (= histidine tag) Epitope tag based on a short stretch (ca 6) of histidine residues. In addition to detection with anti polyhistidine antibodies, such tags permit easy protein purification on nickel based affinity columns … Dictionary of molecular biology
TAP-Tag — Méthode TAP représentation schématique des trois étapes de la purifcation par la méthode TAP La méthode TAP ((en)TAP TAG) est une méthode de purification de complexes protéiques basée sur l utilisation de protéines chimériques portant une … Wikipédia en Français
myc tag — Epitope tag frequently expressed as a translational fusion with a transgenic protein of interest. As there are good antibodies to the myc epitope, this allows localization of the fusion gene product by immunocytochemistry or Western blot, or its… … Dictionary of molecular biology
Thrombin — Coagulation factor II (thrombin) The structure of human thrombin in complex with the inhibitor hirudin.[1] … Wikipedia
