Southern hybridization

Southern hybridization
A procedure in which a cloned, labelled segment of DNA is hybridized to DNA restriction fragments on a Southern blot.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • reverse Southern hybridization — a variation on a Southern blot (q.v.) for detection of specific DNA sequences, in which DNA sequences in solution are first hybridized to a labeled probe and then separated electrophoretically …   Medical dictionary

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  • Southern blot — A Southern blot is a method routinely used in molecular biology to check for the presence of a DNA sequence in a DNA sample. Southern blotting combines agarose gel electrophoresis for size separation of DNA with methods to transfer the size… …   Wikipedia

  • Hybridization probe — In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100 1000 bases long), which is used in DNA or RNA samples to detect the presence of nucleotide sequences (the DNA target) that are complementary… …   Wikipedia

  • Southern blot (blot analysis blot hybridization) — South·ern blot (blot analysis, blot hybridization) (suthґərn) [E.M. Southern, British biologist, born 1938] see under blot …   Medical dictionary

  • Southern Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für die DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA… …   Deutsch Wikipedia

  • Southern blot — A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules. In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis,… …   Medical dictionary

  • Southern blot hybridization — see Southern blot, under blot …   Medical dictionary

  • Southern blotting technique — The procedure used to isolate and identify DNA fragments from a complex mixture. The isolated, denatured fragments are transferred from an agarose electrophoretic gel to a nitrocellulose filter and identified by hybridization with probes …   Dictionary of microbiology

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