cot curve

cot curve
A method to estimate the heterogeneity of sequence of a DNA preparation, based on the observation that the more homogenous the DNA, the more easily (and therefore faster) the annealing of single-stranded DNA will occur. The Cot curve plots the extent of annealing from a fully single-stranded preparation over time. The cot (product of initial concentration and time) at which half the DNA has re-natured is the half-cot, a parameter indicating both the degree of heterogeneity in a complex mixture, and of the extent of complementarity in a mixture of two single-stranded DNA molecules.

Glossary of Biotechnology for Food and Agriculture . 2015.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • Cot curve — Physicochemical technique for measuring the complexity (or size) of DNA. The DNA is heated to make it single stranded, then allowed to cool. The renaturation of the DNA is followed spectroscopically: larger DNA molecules take longer to reanneal …   Dictionary of molecular biology

  • cot — cot1 [kät] n. [Anglo Ind < Hindi khāṭ < Sans kháṭvā] 1. a narrow, collapsible bed, as one made of canvas or plastic sheeting on a frame that can be folded up 2. Brit. a baby s crib cot2 [kät] n. [ME & OE, cottage, hut, lit., covered place;… …   English World dictionary

  • Tautochrone curve — 300px|right|thumb|Four points run over a cycloid from different positions, but theyarrive at the bottom at the same time. The blue arrows show the points acceleration. On the top is the time position diagram.A tautochrone or isochrone curve is… …   Wikipedia

  • Kappa curve — In geometry, the kappa curve or Gutschoven s curve is a two dimensional algebraic curve resembling the Greek letter kappa; (kappa).Using the Cartesian coordinate system it can be expressed as::x^4+x^2y^2=a^2y^2or, using parametric… …   Wikipedia

  • Bell cot — Bell Bell, n. [AS. belle, fr. bellan to bellow. See {Bellow}.] 1. A hollow metallic vessel, usually shaped somewhat like a cup with a flaring mouth, containing a clapper or tongue, and giving forth a ringing sound on being struck. [1913 Webster]… …   The Collaborative International Dictionary of English

  • DNA annealing — The reformation of double stranded DNA from thermally denatured DNA. The rate of reassociation depends upon the degree of repetition, and is slowest for unique sequences (this is the basis of the Cot value; see Cot curve) …   Dictionary of molecular biology

  • reannealing — Renaturation of a DNA sample that has been dissociated by heating. In reannealing the two strands that recombine to form a double stranded molecule are from the same source. Differences in the rate of reannealing led to the early recognition of… …   Dictionary of molecular biology

  • repetitive DNA — Nucleotide sequences in DNA that are present in the genome as numerous copies. Originally identified by the value on the Cot curve derived from kinetic studies of DNA renaturation. These sequences are not thought to code for polypeptides. One… …   Dictionary of molecular biology

  • Trigonometric functions — Cosine redirects here. For the similarity measure, see Cosine similarity. Trigonometry History Usage Functions Generalized Inverse functions …   Wikipedia

  • trigonometry — trigonometric /trig euh neuh me trik/, trigonometrical, adj. trigonometrically, adv. /trig euh nom i tree/, n. the branch of mathematics that deals with the relations between the sides and angles of plane or spherical triangles, and the… …   Universalium

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”